Mining of Tumor-Associated B cells for Tumor Directed Antibodies

Tumors and their associated lymph nodes frequently contain reactive B-cells. We hypothesize that many tumor-associated B-cells encode anti-tumor directed antibodies which may be useful in the design of immunotherapies and in diagnostics. The toolkit may include monoclonal Antibodies (mAb), Antibody-Drug Conjugates (ADCs), Chimeric Antigen Receptor (CAR) T-cell therapy, cancer vaccines and disease screening/staging by emergence of antigen specific circulating antibodies. We use various technologies to capture antibodies encoded by B-cells from tumors and Tumor Draining Lymph Nodes (TDLNs). Our pipeline is expected to facilitate effective and rapid isolation of tumor-directed antibodies, and may further allow tumor associated antigen discovery, and the development of targeted immunotherapies.